Jul 21, 2025 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and …

Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.

In all, 26,855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed …

Primer choice influences the estimated microbial composition. A set of different 16S rRNA gene sequencing primer pairs covering one, two, or three of the variable regions V1 to V9 is commonly …

This specifies the minimal number of bases that the primer must anneal to the template at 5' side (i.e., toward start of the primer) or 3' side (i.e., toward end of the primer) of the exon-exon junction.

Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on exon/intron …

Jun 18, 2012 · We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers. Primer-BLAST offers flexible options to adjust the specificity …

Here, we describe a novel empirical-based method, miPrimer, which greatly reduces primer dimerization and increases primer specificity by factoring various intrinsic primer properties and employing four …

Jan 6, 2025 · High-quality primer design is essential for the success of all polymerase chain reaction (PCR)-based experiments. We previously developed a thermodynamics-based gene-specific …

In this primer, we discuss the ways in which language models, both those based on natural language and those based on biological sequences, can be applied to biological research.